Table 1.

Parameters used to ensure probe sensitivity and specificity

Description	Parameters
Probe length	60 mer
Final orientation	5′→3′
IUPAC	ACTG
% G	<50 %
% GC	≥35 % and  ≤ 55 %
Tm	≥68 and ≤ 76
Homopolymer	≤6
Probe location	3′