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Maximal KD (micromolar) Values for WT, R183K, R183Q and R183A with Ligands Demonstrating Their Varying Affinities at pH 7
| ligand | WT | R183K | R183Q | R183A |
|---|---|---|---|---|
| KCN | 4 | 300 ± 20 | 85 ± 6 | 11000 ± 2000 |
| imidazole | 9.6 ± 0.3 | 18 ± 1 | 27 ± 1 | 21± 1 |
| N3– | 8.3 ± 0.1 | NBa | NBa | NBa |
| F– | 15000 ± 1000 | NBa | NBa |
No binding observed optically up to 400 mM added ligand.
