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. 2012 Jun 7;3:172. doi: 10.3389/fphys.2012.00172

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Copyright © 2012 Ripamonti, Teare and Ferretti.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Induction of bone formation by orthotopic calvarial biphasic hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) harvested on day 30. (A,B) Low and high power views of untreated HA/β-TCP construct showing bone formation pericranially (light blue arrows) (A) within macroporous spaces (A,B). (C,D) Induction of bone formation within concavities (arrows) of the macroporous spaces of untreated HA/β-TCP constructs; the induction of bone forms exclusively within the concavities of the implanted substratum (blue arrows) (E,F) High power views of macroporous spaces treated with 125 μg hTGF-β₃ with multiple osteoblasts surfacing the newly formed bone (dark blue arrows).