Figure 1.

Structure and in vitro analysis of foamy virus vectors.(a) The structure of the foamy virus vectors. Two different internal promoters we used. Wiskott–Aldrich syndrome (WAS) endogenous promoter (Wa0.5) was derived from the 0.5-kb 5′-flanking sequence of the WAS gene for physiological expression. A 631-bp fragment of the UCOE from human HNRPA2B1-CBX3 locus was characterized for completely stable transgene expression. Desired promoter sequences and transgenes are inserted into vector plasmid. (b) WAS protein (WASp) expression in a FV vector-transduced T-cell line established from a WAS patient. Western blot analysis was performed 4 weeks after transduction. CMV, cytomegalovirus; LTR, long-terminal repeat.