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. 2012 Jan 3;20(6):1270–1279. doi: 10.1038/mt.2011.282

Figure 2.

Figure 2

Transduction efficiency in hematopoietic progenitors and expression level of transgene in vivo by single or repeated infection. Lineage marker depleted (Lin-) and Sca-1+ (Lin-/Sca-1+) cells were purified from Was knockout (KO) mice, and transduced with foamy virus (FV) vectors encoding enhanced green fluorescent protein (EGFP) (wE and uE). (a) The percentage of colony-forming unit in culture (CFU-C) colonies positive for EGFP after single transduction [multiplicity of infection (MOI) = 10] and double transduction (MOI = 20) is shown. Flow cytometry (FACS) analysis for EGFP expression was performed in CFU-C colonies after 7-day culture. (b) EGFP expression in peripheral blood T-cells 6 and 12 months after single transduction. (c) Transduction efficiency of uE in Lin-/Sca-1+ cells after repeated infection (MOI = 20 at each cycle). Peripheral blood was analyzed 8 months after transduction. EGFP expression of T cells (CD3), B-cells (B220), granulocytes (Gr1), and platelets is shown.