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. 2012 Jan 3;20(6):1270–1279. doi: 10.1038/mt.2011.282

Figure 4.

Figure 4

Restoration of T- and B-cell function after gene therapy. (a) Splenic T cells were purified with immunomagnetic beads and stimulated with 2 µg/ml anti-CD3 monoclonal antibody (mAB). T-cell receptor (TCR)-driven proliferation was measured by 3H-thymidine incorporation. Results are expressed by the stimulation index (SI), the ratio between cpm of stimulated and nonstimulated cells. Wt n = 7, KO n = 7, wW n = 6, uW n = 6, enhanced green fluorescent protein (EGFP) n = 5. (b) Interleukin (IL)-2 level in conditioned supernatant was measured by ELISA technique. Wt n = 9, KO n = 11, wW n = 6, uW n = 6, EGFP n = 5. (c) Splenic B-cells were incubated in 5-µm transwell and allowed to migrate in response to medium only or medium with 50 µg/ml CXCL12. Results are expressed as the percentage of input cells that migrated. Five mice were analyzed in each group except EGFP (n = 3). Error shown is ±1 SD. *P < 0.05 as compared to KO group, Student t-test.