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. 2012 Feb 14;20(6):1234–1241. doi: 10.1038/mt.2012.24

Figure 3.

Figure 3

Migration inhibitory factor (MIF) expression is upregulated in mesenteric lymph node (MLN) and lamina propria (LP) in dextran sodium sulfate (DSS)-induced colitis. (a) Serum MIF concentration, as measured using an enzyme-linked immunosorbent assay (ELISA) (n = 5 per group). (b) Interleukin (IL)-1β, IL-6, and MIF mRNA expression was determined in MLN and colon specimens using real-time PCR (n = 5 per group). Data were normalized to the expression of β-actin mRNA. (c) Immunohistological staining for MIF in colonic tissue from DSS-treated mice and untreated mice. (d) CD11b+ cells were isolated from LP and MLN in the DSS-treated mice and untreated mice. CD11b+ cells were cultured with 1 µg/ml of LPS. MIF expression was measured at the indicated time point (0, 1, 4, 8, 16, 24, and 48 hours) using real-time PCR and ELISA. The cytokine mRNA expressions were determined in colon and MLN specimens using real-time PCR. Data were normalized to the expression of β-actin mRNA. (e) The supernatants were collected, and the cytokine productions were measured using an ELISA.