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. 2012 Feb 14;20(6):1234–1241. doi: 10.1038/mt.2012.24

Figure 5.

Figure 5

Antisense migration inhibitory factor (MIF)/schizophyllan (SPG) complex inhibited MIF production induced by LPS in vitro. (a) CD11b+ cells from lamina propria (LP) and mesenteric lymph node (MLN) were cultured with several concentrations of antisense MIF/SPG complex (ASMIF), scramble control DNA/SPG complex (SCMIF), and SPG as a control. After 10 hours, 1 µg/ml of LPS was added under each condition and the cells were cultured for 24 hours. MIF expressions were measured using an enzyme-linked immunosorbent assay (ELISA) (n = 5 per group). The data presented are the means of the cytokine concentration ± SD. (b) Immunofluorescence in CD11b+ cells was performed by labeling antisense MIF with TAMRA and the SPG with fluorescein isothiocyanate (FITC). CD11b+ cells took up the antisense MIF/SPG complex effectively compared with the antisense MIF alone. (c) Neutralizing anti-Dectin-1 antibodies inhibited the uptake of FITC-labeled antisense MIF/SPG complex into CD11b+ cells compared with rat IgG2b. The data were presented as the mean of three independent experiments.