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. 2012 Mar 6;20(6):1251–1260. doi: 10.1038/mt.2012.36

Figure 8.

Figure 8

Real-time PCR and western blot analysis detect that Sleeping Beauty (SB) transposon system-based microRNA-29b (miR-29b) gene transfer decreases transforming growth factor-β1 (TGF-β1)/Smad3 signaling to prevent or treat the bleomycin-induced pulmonary fibrosis. (a–d) Gene transfer of miR-29b suppresses the TGF-β1 (c), connective tissue growth factor (CTGF) (d) mRNA level and decreases the Smad3 phosphorylation (a,b) to prevent the bleomycin-induced pulmonary fibrosis at day 14. (e–h) Gene transfer of miR-29b downregulates the mRNA level of (g) TGF-β1, (h) CTGF, and (e,f) decreases the Smad3 phosphorylation to treat the established bleomycin-induced pulmonary fibrosis at day 28. Each bar represents mean + SEM for at least five mice. *P < 0.05, **P < 0.01, ***P < 0.001 versus saline-treated mice; #P < 0.05, versus control vector-treated mice. BLM, bleomycin; CV, control vector.