Figure 4.

The direct effects of androgen on the α₂β₁^hi basal cell differentiation are shown using R1881. Epithelial differentiation of prostatic cells was validated using antibodies specific for prostate acid phosphatase (PAP), prostate specific antigen, cytokeratin 18 (CK18) and androgen receptor (AR). Furthermore, down-regulation of the α₂β₁ integrin was demonstrated. Expression of these differentiation markers was assessed by fluorescence activated cell sorting analysis and data from at least three replicates are summarised in the bar chart. The following controls were used for these experiments: no primary antibody and isotype IgG controls.