Figure 5.

Immunoelectron microscopy of EpiAirway tissues co-cultured for five days with strains 86-028NP or R2866. Following co-culture, most of the intact 86-028NP are paracellular (a, arrows). The bacteria that are intracellular appear to be degraded (boxed area). N = nucleus. (b) Magnified view of the boxed area in (a). Arrows indicate the intracellular and degraded bacteria, which react with the anti-NTHi antiserum. N = nucleus. (c) Paracellular organisms of strain R2866. Arrows indicate intact bacteria; arrowhead indicates an outer membrane vesicle (OMV) that reacts with anti-NTHi antiserum. NTHi, non-typeable Haemophilus influenzae