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. Author manuscript; available in PMC: 2012 Jun 7.
Published in final edited form as: Methods Mol Biol. 2012;818:217–226. doi: 10.1007/978-1-61779-418-6_15

Fig. 2.

Fig. 2

Analysis of Fus1-Apaf-1 interaction using synthetic peptides designed from predicted protein interaction motifs by an ethanolamine protection assay on a ProteinChip array with SELDI-MS. The specific interaction of the Fus1 PDZ domain with Apaf-1 c-terminal peptide was detected as indicated by accurate mass of each peptide and by comparison with negative mutant and nonspecific control peptides. (a) Panels show individual peaks of various Fus1 or Apaf1 peptides when single peptides were loaded onto proteinchip arrays; (b ) two distinctive peaks are detected only when the peptides specifically interact with each other; and (c) when a mutation is introduced in the PDZ binding motif of Fus1, no Apaf1 peptide peak appears in the spectrum.