dAd5GNE efficacy in mice. A. Anti-cocaine IgG antibody titers over time. BALB/c mice (n=20) were vaccinated intramuscularly with 4 µg dAd5GNE + Adjuplex at 0, 3 and 6 wk. Antibody titers were assessed by ELISA at 0, 3, 8, 10 and 23 wk. The control was unconjugated dAd5LacZ. B. Cocaine-induced locomotor activity was assessed upon 6 cocaine exposures over a 3 wk period. All test groups were initially assayed with PBS to establish baseline activity (shown by non-connected symbols at day 0). Mice were sensitized to cocaine (15 mg/kg, i.p.) during the 1st wk of the trial (days 1–4), and then challenged weekly (n=10/group).