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Multiplexed dye delivery. (a) Calcein uptake was induced in the vertical bar area (green channel). (b) Dextran-tetramethylrhodamine uptake was induced in the horizontal bar area (yellow channel). (c) Delivery for both calcein and rhodamine was induced in the 4 corners of the square pattern in the cell culture. (d) Phase contrast image shows a confluent monolayer of viable HeLa cells that were imaged by immunofluorescence microscopy in panels (a-c). Bar = 100 µm.
