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. 2012 Apr 12;119(22):5144–5154. doi: 10.1182/blood-2011-07-368506

Figure 6.

Figure 6

Cx43 regulates the cellular composition in nonmyeloablated BM. (A-B) Content of BM mesenchymal (CFU-F; A) and osteoblastic progenitors (CFU-OB; B) in BM of WT and OB/P Cx43-deficient mice. (C-E) Quantification of immunophenotypically identified nonhematopoietic cells (C) and cadherin-11 negative (D) or positive (E) subpopulations. (F-G) OB/P-Cx43–deficient mice showed an increased number of Cxcl12+ cells among both CD45−/Ter119−/cadherin-11+ and CD45−/Ter119−/cadherin-11− cell subpopulations. (H) Representative example of flow cytometry analysis of 5-bromo-2'-deoxyuridine (BrdU+) cells on different mesenchymal cell subpopulations. (I) In vivo proliferation of BM CD45−/Ter119− and Cxcl12+ mesenchymal cells. (J) Normalized RNA expression of sclerostin in cortical bones from WT or OB/P Cx43-deficient mice. Results for sclerostin expression are presented as average of 2 mice per group where expression was analyzed in femora, tibiae, and pelvic cortical bones. Empty bars present control data; solid bars represent data from OB/P Cx43 deficient mice. Analysis of OB/P populations are shown as mean ± SD. N = 3 different experiments n = 6 mice per group (*P < .05; **P < .01).