. 2012 Mar 7;119(22):5155–5163. doi: 10.1182/blood-2011-11-388918

Table 2.

Treg expression of CTLA-4 is required for in vivo suppression of autoreactive T cells

Unirradiated host	CD45RB^hi donor T cells	Donor CD4⁺CD25⁺ Tregs		Survival at 18 wk
Unirradiated host	CD45RB^hi donor T cells	Mouse origin	Genetic origin	Survival at 18 wk
RAG-2^−/−	B6	None	None	0/4
RAG-2^−/−	B6	B6+Ctla4^−/−→B6	B6^CD45.1	4/4
RAG-2^−/−	B6	B6+Ctla4^−/−→B6	Ctla4^{−/−CD45.2}	0/4

RAG-2^−/− mice were injected with CD4⁺CD45RB^high (B6) cells either alone or together with sorted CD4⁺CD25⁺ donor cells of B6 or Ctla4^−/−origin that were isolated from B6+Ctla4^−/−→B6 mixed chimeras. Mice were observed daily and their survival at 18 weeks is noted.