Skip to main content
. 2012 Apr 4;119(22):5239–5249. doi: 10.1182/blood-2011-12-398362

Figure 3.

Figure 3

Pu.1 initiates the expression of runx1, whereas Runx1 suppresses late-phase expression of pu.1. (A-B) WISH of pu.1 expression in 17.5 hpf sib (A) and runx1w84x (B) embryos. Arrows indicate WISH signal of pu.1. (C-D) Whole-mount antibody staining of Pu.1 protein in 17.5 hpf sib (C) and runx1w84x (D) embryos. Arrows indicate fluorescent antibody staining signal for Pu.1 protein. (E-F) WISH of runx1 expression in 17 hpf control (E) and pu.1 morphants (F, n = 47 of 48). The arrow indicates WISH signal of runx1 in control morphants (E), which is absent in pu.1 morphants (F). (G-H) WISH of runx1 expression in 17 hpf sib (G) and pu.1G242D mutants (H). Arrows indicate WISH signal of runx1, which is markedly reduced in pu.1G242D mutants (H) compared with sib (G). (A-H) Embryos are viewed dorsally with the anterior to the left.