Figure 3.

Effect of varying the concentrations of PF4 and heparin on the induction of TF expression in PBMCs. (A) Increasing amounts of heparin were added to PBMCs containing 100 μg/mL KKO and 10 μg of PF4. Samples were incubated for 6 hours at 37°C, and TF activity was measured with a one-stage clotting assay. (B) Increasing amounts of PF4 were added to PBMCs containing 100 μg/mL KKO and 1 U/mL heparin. TF activity was measured with a one-stage clotting assay. Results are from 3 independent experiments.