Figure 5. Image rotation confirms localization of Kir6.2 in striatal DAergic axons.

A. Representative aligned superimposed fields from a section of guinea-pig striatum immunostained using C62 anti-Kir6.2 with anti-TH. In all panels, TH-ir profiles are white; Kir6.2-ir puncta that colocalize with a TH-ir profile are gray; Kir6.2-ir puncta that do not colocalize with TH-ir profiles are black. B. Rotation of the Kir6.2-ir image from (A) 90° clockwise markedly decreased the degree of colocalization. C,D. Another example of aligned and rotated merged immunostained images using NBP1-00900 anti-Kir6.2 with anti-TH. Scale bar in (A) is 10 μm and applies to all panels. See Table 1 for quantitative analysis of several superimposed aligned and rotated fields. E. Plot of Kir-6.2-ir density versus the percent occupancy of the field of TH-ir processes from 6 separate striatal sections is positively correlated (r² = 0.49) providing further evidence for colocalization.