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Expression of pluripotency markers in embryonic stem cells (ESCs) derived from blastocysts produced by in vitro fertilization (IVF), parthenogenesis, or hand-made cloning (HMC). Lane 1, 100-bp ladder; lane 2, IVF; lane 3, parthenogenesis; lane 4, HMC; lane 5, PCR −ve); lane 6, reverse transcriptase −ve.
