Table 1.
Developmental Competence and Quality Assessment of Buffalo Embryos Produced by In Vitro Fertilization, Parthenogenesis, and Hand-Made Cloning
Source | No. of oocytes/reconstructed embryos (n) | Cleavage rate n (%) | Blastocyst rate n (%) | Total cell number | ICM cell number | ICM/TE ratio |
---|---|---|---|---|---|---|
IVF | 1250 | 898 (70.23±3.34)a | 112 (12.13±1.62)a | 158.50±6.80a | 26.50±2.26a | 0.20±0.02a |
Parthenogenesis | 622 | 545 (81.09±2.34)b | 153 (24.60±2.29)b | 169.83±11.99a | 27.66±4.05a | 0.19±0.02a |
HMC | 296 | 238 (86.94±3.90)b | 142 (48.18±3.63)c | 231.33±15.72b | 43.50±1.98b | 0.24±0.03a |
Values with different superscripts within the same column differ significantly (p<0.05).
ICM, inner cell mass; TE, trophectoderm; IVF, in vitro fertilization; HMC, hand-made cloning.