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Table 3.

Effect of LIF on the Primary Colony Formation Rate Following Seeding of ICMs Obtained from Blastocysts Produced by In Vitro Fertilization, Parthenogenesis, and Hand-Made Cloning

	Source
	Primary colony formation rate (%)
LIF concentration (U mL⁻¹)	IVF	Parthenogenesis	HMC
0	29.98±2.33^a	33.575±2.49^a	28.51±1.57^a
1000	32.16±1.44^a	35.79±2.76^a	30.64±1.24^a
2000	24.15±2.30^a	26.59±2.65^a	20.68±1.57^{a^*}
4000	12.36±2.69^{a^*}	9.26±1.67^{a^*}	8.835±1.78^{a^**}

Values with different superscripts within the same row differ significantly (p<0.05).

^{^*}

Indicates significant difference within the same columns (p<0.05).

LIF, leukemia inhibitory factor; ICM, inner cell mass; IVF, in vitro fertilization; HMC, hand-made cloning.