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. 2012 Jun;14(3):225–234. doi: 10.1089/cell.2011.0069

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 2. — Representative light micrographic overviews of porcine IV zygote (A1), two-cell embryo (B1), four-cell embryo (C1), and eight-cell/morula stage (D1). Arrowheads indicate pronuclei and nuclei. (A2–D2) Transmission electron micrographs of pronuclei (zygote) and nuclei (embryos). Arrows indicate heterochromatin loose in the nucleoplasm or attached to the nuclear envelope. Arrowheads indicate heterochromatin clusters attached to the nucleolus precursor bodies (NPBs). dN, developing nucleolus; FGN, fibrillogranular nucleolus. (A3–D3) Transmission electron micrographs of NPBs/nucleoli in zygote (A3) and embryos (B3–D3). Arrowheads indicate heterochromatin clusters attached to the NPBs. Note the formation of fibrillar centers (FC), dense fibrillar component (DFC), and granular component (GC) around the NPB in the four-cell embryo (C3) and the presence of a fibrillogranular nucleolus with the same components in the eight-cell/Ml stage (D3).