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. 2011 Apr 26;2(7):370–378. doi: 10.1021/cn200032r

Figure 5.

Figure 5

Specific SavQdot-IDT444 labeling of DAT stably expressed in living HeLa cells in situ. Representative confocal images are shown for DAT-pcDNA3 transiently transfected HeLa cells (A1,A2), DAT-pcDNA3 transiently transfected cells pretreated with 1 μM GBR12909 (B1,B2), and sham-pcDNA3 transiently transfected cells (C1,C2). Fluorescent (top) and overlay (bottom) images are shown. All samples were incubated with 100 nM solution of IDT444 in the imaging buffer for 5 min at 37 °C and subsequently exposed to 1 nM SavQdot in the imaging buffer for 2–3 min at 37 °C. Images are representative of at least five independent experiments.