Figure 10.

Flemish Aβ(1–40) predominately forms a variety of oligomeric aggregates on mica. (A) Freshly prepared Flemish Aβ was imaged continuously in solution on mica at a concentration of 40 μM. The increased concentration was used because the Flemish aggregation was much slower compared to Wild Type and the other mutants. 5 × 5 μm² images are presented as 3D reconstructions with indicated zoomed in 1 × 1 μm² areas presented in 2D. Yellow arrows indicate two oligomers coalescing into a slightly larger oligomer. The blue arrows indicate short, slightly elongated aggregates that were stable. (B) 2D Fourier analysis performed on a representative image (shown to the upper left) demonstrated that Flemish aggregates did not display long-range order along the mica surface. (C) Height profiles of Flemish Aβ aggregates on mica (corresponding to the lines indicated in the adjacent images) and (D) height histograms of all aggregates after 362 min of aggregation, demonstrating that Flemish Aβ formed a variety of oligomers of different heights.