| Medium to prepare | Reagents | Quantity |
| "DMEM incomplete" | DMEM-F12 Ham | 500ml |
| L-glutamine 200mM | 5ml | |
| Penicillin/streptomycin 100X | 5 ml | |
| NaOH 1M | 1.3 ml (adjust pH to 7.4) | |
| "DMEM complete" | "DMEM incomplete" | 450ml |
| Foetal Bovine Serum heat-inactivated | 50ml | |
| Endothelial cell growth supplement | 5 ml |
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