| Medium to prepare | Reagents | Quantity |
| "RPMI incomplete" | RPMI 1640 (with bicarbonate) | 500ml |
| Hepes 1M | 12.5ml | |
| Glucose 20% | 5ml | |
| L-glutamine 200mM | 5ml | |
| Gentamycin 50mg/ml | 250μl | |
| NaOH 1M | 0.7 ml (adjust pH to 7.2) | |
| "RPMI complete" | "RPMI incomplete" | 450ml |
| Pooled human (non-immune) serum | 50ml |
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