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. 2012 Jan 3;(59):3122. doi: 10.3791/3122
Name of the reagent Company Catalogue number Comments
DMEM-F12 Ham Sigma D6421 For DMEM complete medium
L-glutamine 200mM GIBCO 25030 For DMEM and RPMI complete medium
Penicillin/streptomycin 100X (10000 units/ml and 10mg/ml) ScienCell 0503 For DMEM complete medium
Foetal Bovine Serum heat-inactivated ScienCell 0025 For DMEM complete medium
Trypsin-EDTA (0.025% Trypsin,  0.5mM EDTA) ScienCell 0103  
Endothelial cell growth supplement ScienCell 1052 For DMEM complete medium
Tissue culture treated 60 mm X 15 mm Petri dishes BD 353002  
Human Fibronectin Millipore FC010 Use at 2 μg/cm2
TNF R&D Systems 210-TA optional, use at 50 μg/ml
RPMI 1640 Lonza BE12-167F For RPMI complete medium
Gentamycin 50mg/ml Lonza 17-518Z For RPMI complete medium
Hepes 1M Lonza BE17-737E For RPMI complete medium
HDMEC ScienCell 2000 Primary cell line
HPMEC ScienCell 3000 Primary cell line
HBEC-5i     Obtained from Francisco Candal (fcandal@cdc.gov)