Name of the reagent
|
Company
|
Catalogue number
|
Comments
|
DMEM-F12 Ham |
Sigma |
D6421 |
For DMEM complete medium |
L-glutamine 200mM |
GIBCO |
25030 |
For DMEM and RPMI complete medium |
Penicillin/streptomycin 100X (10000 units/ml and 10mg/ml) |
ScienCell |
0503 |
For DMEM complete medium |
Foetal Bovine Serum heat-inactivated |
ScienCell |
0025 |
For DMEM complete medium |
Trypsin-EDTA (0.025% Trypsin, 0.5mM EDTA) |
ScienCell |
0103 |
|
Endothelial cell growth supplement |
ScienCell |
1052 |
For DMEM complete medium |
Tissue culture treated 60 mm X 15 mm Petri dishes |
BD |
353002 |
|
Human Fibronectin |
Millipore |
FC010 |
Use at 2 μg/cm2
|
TNF |
R&D Systems |
210-TA |
optional, use at 50 μg/ml |
RPMI 1640 |
Lonza |
BE12-167F |
For RPMI complete medium |
Gentamycin 50mg/ml |
Lonza |
17-518Z |
For RPMI complete medium |
Hepes 1M |
Lonza |
BE17-737E |
For RPMI complete medium |
HDMEC |
ScienCell |
2000 |
Primary cell line |
HPMEC |
ScienCell |
3000 |
Primary cell line |
HBEC-5i |
|
|
Obtained from Francisco Candal (fcandal@cdc.gov) |