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. 2011 Nov 14;3(2):129–140. doi: 10.1021/cn200109w

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Representative Western blot demonstrating the ratio of MB to S-COMT in blood (lanes 1–2), brain (lanes 3–4), or liver (lanes 5–6) fractions utilized in the COMT ex vivo assay from male Wistar rat. To demonstrate similar immunoreactivity for rat MB versus S-COMT, equal amounts of recombinant rat MB (lane 7) or S-COMT (lane 8) were also resolved. Immunoblotting with COMT antibody (Santa Cruz-135872, dilution 1:200) revealed immunoreactivity for MB (indicated by upper arrow) and S-COMT (lower arrow).