Figure 1.
NPY effects on cell viability in cultured rat cortical neurons exposed to Aβ25–35. Cortical neurons were preincubated alone (a) or with three concentrations of NPY [(b) 2 mM, (c) 1 mM, and (d) 0.5 mM] for 24 h and then exposed to Aβ25–35 (50 μM) or Aβ35–25 (50 μM) for 48 h (number of experiments performed = 4). After 24 and 48 h, cells were tested with MTS assay. NPY treatment restored normal cell survival. Cell viability is expressed as percent of the mean absorbance value of untreated cells at each time point. Data represent means ± SEM