Table 3. Sequences of the primers designed for PCR amplification and sequencing of the WISP3 gene.
Primer Name | Primer Sequence | Size(bp)/AT(°C) |
WISP3-2F | 5′ TGCTCTCCAGGAACAGGTAAC 3′ | 483 bp/56.6°C |
WISP3-2R | 5′ CTTCCTCCATCTTCGTTTTG 3′ | |
WISP3-3F | 5′ CCTGTTTGGGGGAAATCTTCT 3′ | 531 bp/55.8°C |
WISP3-3R | 5′ TACAATGGAGCCAGTCCCACT 3′ | |
WISP3-4F | 5′ TCCTGTGAAGGAGGTTCCAAA 3′ | 553 bp/54.9°C |
WISP3-4R | 5′ TCCCTGTCTGAGGCAAAGATT 3′ | |
WISP3-5F | 5′ AGGCAAAGCAGAAAAATGCAA 3′ | 559 bp/51.7°C |
WISP3-5R | 5′ ATCCCACCCTCCAAAACACAC 3′ | |
WISP3-6F | 5′ AAGGGTAAAGAGAGTGCTGGA 3′ | 518 bp/51°C |
WISP3-6R | 5′ AAACAAAGTAGATTTGCCACCA 3′ |
“AT”: Anneal Temperature.