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. 2012 Jun 7;7(6):e38566. doi: 10.1371/journal.pone.0038566

Figure 5. EphA3 ectodomain decreases the density of interstitial filopodia in nasal RGC axons.

Figure 5

A–D. Representative microphotographs of axons grown from nasal (A, C) and temporal (B, D) retinal explants exposed to soluble clustered Fc (A, B) or EphA3-Fc (C, D). Axons are labeled with Alexa 488-phalloidin. Arrows depict representative interstitial filopodia. Insets show filopodia at higher magnification. Scale bars  = 20 µm. (E) Quantification of filopodia number/100 µm of axon shafts. Nasal axons present higher density of interstitial filopodia and EphA3-Fc significantly decreases the density of interstitial filopodia in nasal RGC (ANOVA and Tukey postest, 3 experiments, n: 8 axons for explant, 4 explants for condition). Results are shown as mean +/− SE.