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. 2012 Jun 7;7(6):e38385. doi: 10.1371/journal.pone.0038385

Figure 6. Comparative expression analysis of cruzipain and homologues in T. cruzi, T. cruzi marinkellei, T. dionisii and T. rangeli.

Figure 6

(A) Northern blotting analysis of cruzipain transcripts from T. cruzi (Y) and cross-hybridization using the probe consisting of PCR-amplified catalytic domains of T. cruzi Y cruzipain labeled with 32P T. cruzi Y (TcY-CatL probe); agarose gel of RNA used for this analysis was stained with ethidium bromide (EtBr). (B) CATL proteolytic activities detected in epimastigote lysates of T. cruzi G, T. cruzi marinkellei, T. dionisii and T. rangeli. Activity banding profiles detected in gelatin gels, pH 5.0 and 5 mM DTT were inhibited in gel incubated with 10 µM E-64.