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. Author manuscript; available in PMC: 2012 Jun 7.
Published in final edited form as: Angiogenesis. 2010 Nov 21;14(1):47–59. doi: 10.1007/s10456-010-9194-9

Fig. 1.

Fig. 1

Broad spectrum protease inhibition reveals that ASC-mediated vessel invasion is plasmin dependent, while MMPs are required in regulating vessel diameter. HUVECs, transduced with mCherry, were coated on microcarrier beads and cultured within 2.5 mg/mL fibrin gels in the presence of ASCs. (a) Representative day 7 images of vessel formation in the presence of DMSO vehicle control, 10 μM GM6001, 10 mM EACA, or GM6001 + EACA are shown. Total network length (b) and average vessel width (c) were calculated and normalized to the vehicle control. ***, **, and * refer to p ≤ 0.001, p ≤ 0.01, and p ≤ 0.05 in comparison to the vehicle control, respectively. # refers to p ≤ 0.05 in comparison to the EACA condition. Scale bar = 100 μm