Table 2. Primer Sequences Used.

Gene	Primer Sequences (5′-3′)	PCR product (bp)	Annealing temp (°C)
ADM	F: CGAAAGAAGTGGAATAAGTGGG	200	60
	R: GTTCATGCTCTGGCGGTAGCG
CRLR	F: GCTGAATGACGTTGCAGC	480	60
	R: GCCTTCACAGAGCATCCA
RAMP2	F: CATGGACTCTGTCAAGGACTG	153	55
	R: GTGTATCAGGTGAGCCT
RAMP3	F: ACCTGTCGGAGTTCATCG	262	55
	R: ATCAGTGTGCTTGCTGCG

Primer sequences used in this study for RT–PCR (ADM, CRLR, RAMP2, RAMP3).