Table 1. Pichia pastoris strains generated for systematic investigation of the relationship between protein complexity, gene dosage, relative expression and secretion.

Strain Namea	Secreted protein	Gene copyb number	Relative mRNAc Expression
pGAPαeGFP1	eGFP	1	2.1
pGAPαeGFP2	eGFP	3	12.7
pGAPαeGFP3	eGFP	≥4	19.4
pGAPαGFc1	Glycosylated Fc	1	1
pGAPαGFc2	Glycosylated Fc	2	1.4
pGAPαGFc3	Glycosylated Fc	≥6	11.3
pGAPαAgFc1	Aglycosylated Fc	1	5.1
pGAPαAgFc2	Aglycosylated Fc	2	5.4
pAOXαeGFP1	eGFP	2	16.4
pAOXαeGFP2	eGFP	5	19.8
pAOXαGFc1	Glycosylated Fc	2	10.9
pAOXαGFc2	Glycosylated Fc	5	8.9
pAOXαAgFc1	Aglycosylated Fc	2	5.1
pAOXαAgFc1	Aglycosylated Fc	4	22.2

^aStrain names indicate promoter used.

^bDetermined by qPCR using an absolute quantification of transcript copy number.

^cDetermined by RT-qPCR using an absolute quantification of transcript copy number and the expression of actin as a control.