Figure 2. Recruitment of IRF3, IRF7 and TBP to IFN-A gene promoters.

Recruitment to the IFN-A2, A14 and A1 gene promoters was determined by ChIP-QPCR assays in Namalwa B cells infected by Sendai virus. Cross-linked chromatin extracts were immunoprecipitated with antibodies specific for TBP, IRF7 or IRF3 and submitted to QPCR analysis after reversion of cross-linking, as described in Materials and Methods. Anti-rabbit IgG was used to determine background levels of non-specific binding. Data are expressed as % of the DNA input. Values are mean and standard deviation for two replicate samples derive from two representative experiments (n = 4). The error bars indicate the relative standard deviations.