Figure 4. Recruitment of HDAC3 to IFN-A gene promoters during virus infection.

(A) Relative expression levels of HDAC3 were determined in Sendai virus-infected Namalwa cells by RT-QPCR as described in Figure 1. HDAC3 protein levels were analyzed in whole cell extracts prepared from Sendai virus-infected Namalwa B cells by Western blotting using HDAC3 and actin antisera. (B) Recruitment of HDAC3 and HDAC1 to the IFN-A2, A14 and A1 gene promoters was determined in Namalwa B cells infected by Sendai virus for 4 to 16 h by quantitative ChIP assays as described in Figure 2. Anti-rabbit IgG was used to determine non-specific binding.