Figure 5. Regulation of histone H3 acetylation associated with the IFN-A2 gene promoter.

(A) IFN-A2 expression was determined in HEK293-TLR3 cells co-expressing IKKε and IRF7 alone or together with IRF3. Cells were collected for RT-QPCR analysis at the indicated times (in hours) after transfection. (B) Acetylated histone H3K9, acetylated H3K14 and histone H3, as well as recruitment of IRF3, IRF7 and HDAC3 associated with the IFN-A2 gene promoter were determined by quantitative ChIP assays as described in Materials and Methods. Anti-rabbit IgG was used to determine non-specific binding.