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. 2012 Jun 7;7(6):e37920. doi: 10.1371/journal.pone.0037920

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Mitchell et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A highly sensitive assay of RD1-specific IFN-γ production using real time quantitative PCR (qPCR) for two reporters - monokine-induced by IFN-γ (MIG) and the IFN-γ inducible protein-10 (IP10) was compared longitudinally with the ex vivo RD1-Elispot. Figure 3A: A head-to–head comparison was performed on 14 individuals on samples from 2 time-points 3 months apart. A significant correlation was observed between fold change on the RD1 qPCR and change in SFC using the RD1-Elispot. Figure 3B: Data from SK068. Figure 3C: Data from SK036. Top panel displays RD1-Elispot data and bottom-panel displays RD1-qPCR data.