Figure 3. Decreased mtDNA Mutagenesis in Colorectal Carcinoma Is Attributable to a Reduction in C∶G to T∶A Transitions.

Mitochondrial DNA mutation spectrum (± s.e.m.) per Mb in human colorectal tissue. Mutant mtDNA amplicons (n = 796) spanning two restriction sites (1215–1218 and 7335–7338) were recovered from the RMC assay and subjected to DNA sequencing to generate the mutational signature of mtDNA isolated from normal (n = 297), adenoma (n = 275), and carcinoma (n = 224) colorectal tissues. * P<0.0001; two-tailed paired t-test.