Figure 8. Responsiveness of the ef0120 leader region to regulation by EutVW.

A) One outcome of the covariance searches was identification of a new putative ANTAR hit within the E. faecalis genome. This hit contained an overlapping intrinsic terminator hairpin, as identified by TransTermHP, despite the fact that a terminator was not a stringent requirement of the initial search criteria. B) To test whether this hit was functionally responsive to EutVW in vivo, the leader region of this gene was translationally fused to a lacZ reporter and monitored with and without AdoCbl and ethanolamine.