Figure 7. mir93 promotes MET in normal breast epithelial cells.

A. Single cells were isolated from normal breast tissues and stained with EpCAM-APC and CD49f-FITC for FACS sorting. After sorting, total RNA were isolated from different sorted groups (EpCAM⁺CD49f⁻, EpCAM⁺CD49f⁺, EpCAM⁻CD49f⁺, EpCAM⁻CD49f⁻) and mir-93 expression were measured by qRT-PCR. p<0.05; Error bars represent mean ± STDEV. B. Single cells were isolated from normal breast tissues and infected with mir-93-expressing lentiviruses in suspension. After one week, mammospheres were dissociated into single cells and plated in adherent culture in the absence (CTRL) or presence (DOX) of DOX for two weeks. Then, cells were dissociated and stained with EpCAM-APC and CD49f-FITC for FACS analysis. C. MCF10A cells were cultured in the absence (CTRL) or presence (DOX) of DOX for two weeks. Cells were then dissociated and stained with EpCAM-APC and CD49f-FITC for FACS analysis.