Table 1. Quantification of structures identified in 3D reconstructions of VZV infected host cell nuclei.
Figure/Object | Volume ( µm3) | Number of all capsids | Number of free capsids | Number of sequestered capsids | Capsid density (capsids/µm3) |
Figure 3A–E/Nucleus 1 | 95 | 4,223 (100%) | 4,223 (100%) | 0 | 44.4 |
Figure 3F/Nucleus 2 | 46.2 | 425 (100%0 | 425 (100%) | 0 | 9.2 |
Figure 3G/Nucleus 3 | 43.4 | 1,340 (100%) | 1,340 (100%) | 0 | 30.9 |
Figure 4B–D/Nucleus 4 | 63 | 3,062 (100%) | 6 (0.2%) | 3,056 (99.8%) | 48.6 |
Figure 4B–D/Cage 1 (top) | 6.2 | 1,732 | 279.4 | ||
Figure 4B–D/Cage 2 (bottom) | 4.6 | 1,324 | 287.8 | ||
Figure 5A–G/Nucleus 5 | 291 | 5,597 (100%) | 70 (1.3%) | 5,527 (98.7%) | 19.2 |
Figure 5A–G/Cage 1 | 0.8 | 126 | 157.5 | ||
Figure 5A–G/Cage 2 | 10 | 2,780 | 278 | ||
Figure 5A–G/Cage 3 | 5.8 | 1,778 | 306.5 | ||
Figure 5A–G/Cage 4 | 3.3 | 843 | 255.5 | ||
Average capsid packing density of PML cages 1–4 of nucleus 5 (capsids/µm3) ± SD | 249±64 (N = 4) | ||||
Average capsid packing density of all reconstructed PML cages (capsids/µm3) ± SD | 261±53 (N = 6) | ||||
Average capsid density in all reconstructed nuclear volumes (capsids/µm3) ± SD | 30±17 (N = 5) |
The number of free or sequestered nucleocapsids identified in VZV infected host cell nuclei and the volume of the analyzed nuclei and PML cages were determined by counting the corresponding traces in all serial sections that were used to generate the 3D models. Capsid densities were calculated by dividing the number of nucleocapsids with the corresponding volume (µm3) of the PML cage or nucleus. The left column indicates the corresponding figures where the quantified objects are visualized.