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. 2012 Jun 7;8(6):e1002732. doi: 10.1371/journal.pgen.1002732

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Agarwala et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) m⁶A accumulation on mRNA was quantified in rich conditions in wild-type (SAy821), ime4Δ/Δ (SAy771), P_CUP1-IME4 (SAy1249), P_CUP1-MUM2 (SAy1251), P_CUP1-SLZ1 (SAy1250), P_CUP1-IME4 P_CUP1-MUM2 P_CUP1-SLZ1 (SAy1248) and P_CUP1-IME4 P_CUP1-IME4 (SAy1252) after 150 minutes of mitotic growth in the presence of cupric sulfate. B) Western analysis for expression of Ime4 and Mum2. Cells encoding epitope-tagged Ime4 and Mum2 (SAy1232) were collected either from the rich cupric-sulfate media conditions in (A) (first column) or at 0, 2, and 5 hours in meiosis (as labeled), then subjected to Western analysis for either Ime4 (anti-myc) or Mum2 (anti-HA). Pgk1 serves as a loading control. Images across each row come from the same exposure.