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. 2012 Jun 7;8(6):e1002732. doi: 10.1371/journal.pgen.1002732

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Agarwala et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Quantification of m⁶A abundance relative to cytosine (blue bars, left axis) and budding index (green triangles, right axis) upon RTG₃. B) Western analysis for 3x-myc-tagged Ime4 protein (SAy914), 3x-HA-tagged Mum2 protein (SAy1235) or 3x-HA-tagged Slz1 protein (SAy1254) throughout RTG₃ (i.e., following the shift to YPD after 3 hours in SPO); Pgk1 protein serves as loading control. C) Representative images of cells from wild-type (SAy821), ime4Δ/Δ (SAy771) and a strain induced to express the three components, IME4, MUM2 and SLZ1 (SAy1248) from P_CUP1 after RTG₃. All strains were treated with cupric sulfate upon RTG₃ into YPD. D) Axial ratio quantifications of RTG₃ cells from cells in (C) (n = 200 cells/strain).