Figure 4. Transcription quantification of ZC3H11A, SLC30A10, and LYPLAL1 in mouse retina, retinal pigment epithelium, and sclera in induced myopic eyes, fellow eyes, and independent control eyes.
Myopia was induced using −15 diopter negative lenses in the right eye of mice for 6 weeks. Uncovered left eyes were served as fellow eyes and age-matched naive mice eyes were controls. Quantification of mRNA expression in mice neural retina (retina), retinal pigment epithelium (RPE) and sclera using quantitative real-time PCR. The bar represents the fold changes of mRNA for each gene after normalization using GAPDH as reference. The mRNA levels of murine ZC3H11A, a gene that is conserved with respect to ZC3H11B in human, SLC30A10 and LYPLAL1 in myopic and fellow retina, RPE and sclera are compared with independent controls with P-values as follows: ZC3H11A (retina/RPE/sclera, P = 2.60×10−5, 2.62×10−6 and 1.08×10−4 respectively), SLC30A10 (P = 2.00×10−4, 2.00×10−4 and 4.02×10−4 respectively) and LYPLAL1 (P = 1.50×10−4, 1.50×10−4, 1.54×10−4 respectively). *P<0.0001.