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Quantification of EPCs by flow cytometry. Circulating EPCs were identified by flow cytometry with low cytoplasmic granularity (R1) and with expression of cell surface antigens such as CD45lowCD34+ VEGFR2+. Arrows indicate CD45lowCD34+ VEGFR2+ cells. EPCs: endothelial progenitor cells, VEGFR2: vascular endothelial growth factor receptor 2, SSC: side scather, FITC: fluorescein isothiocyanate.
