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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: J Immunol. 2012 May 11;188(12):6300–6308. doi: 10.4049/jimmunol.1200256

Figure 1.

Figure 1

LIF expression and biological activity in the lungs during pneumonia. (A) LIF protein concentrations were quantified in bronchoalveolar lavage fluid (BALF) 0–24 hrs after intratracheal (i.t.) instillation of Escherichia coli. Values are expressed as means ± SEM (n = 4–5). * p < 0.05 compared to uninfected (0h) controls. (B) Immunohistochemistry was used to visualize Y705-phosphorylated STAT3 in histological lung sections prepared from mice treated for 1h with or without i.t. recombinant murine LIF (rmLIF). Background staining was undetectable on sections from i.t. rmLIF mice exposed to an isotype control antibody (not shown). Representative images are shown at 10× and 20× magnification. (C) LIF protein concentrations were quantified in BALF 24 hrs after i.t. E. coli co-instilled with anti-LIF or control IgG. Values are expressed as means ± SEM. * p < 0.05 compared to mice treated with control IgG (n = 4–5). (D) Y705- phosphorylated STAT3 immunoreactivity was measured by immunoblot in lung homogenates collected from mice 1h after i.t. rmLIF in the presence of 0–10 µg anti-LIF.