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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: J Immunol. 2012 May 11;188(12):6084–6092. doi: 10.4049/jimmunol.1200368

Figure 1. Differential Rag1 transcription occurs within pro-B cells at different phases of the cell cycle as a result of fluctuations in IL-7 responsiveness.

Figure 1

(A) Wild-type bone marrow pro-B cells were analyzed for IL-7R expression and cell cycle stage using flow cytometry. CD19+/IgM-/c-kit+ pro-B cells were divided into G0/G1 and S/G2/M by DAPI staining, and back gated for either IL-7R (blue) or an isotype (IG) control (red). (B) Wild-type bone marrow pro-B cells cultured short-term with 5ng/ml of IL-7 in vitro were analyzed for phospho-STAT5 levels and cell cycle stage using flow cytometry. Cells were divided into G0/G1 and S/G2/M by DAPI staining, and back gated for either phospho-STAT5 (blue) or an isotype (IG) control (red). (C) Phospho-STAT5 levels were analyzed across cell cycle stages within v-Abl transformed pro-B cells as described in (B).

(D), (E) Graphs showing the level of Rag1 and Iμ transcripts assessed by Q-PCR (lower panels) in sorted G0/G1 and S/G2/M bone marrow pro-B cells cultured short-term in the presence of IL-7 (5ng/ml) (D) or v-Abl pro-B cells (E) (upper panels). Transcripts were normalized against β2 microglobulin and the G0/G1 population set at 1. Each data set is representative of 3-4 experiments.